Journal: Cell

Article Title: Citrate clearance is a major function of aconitase 2 in the canonical TCA cycle

doi: 10.1016/j.cell.2026.01.028

Figure Lengend Snippet: (A and B) Citrate concentration in serum (A) and citrate levels in tissues (B) from indicated mice 10 days post tamoxifen administration (serum: n = 11 mice/genotype; other tissues: n = 5–11 mice/genotype). (C and D) Representative hematoxylin & eosin (top) and immunohistochemistry for NGAL (bottom) in kidneys from indicated mice 20–25 days post tamoxifen administration (C). Black arrows indicate areas of renal tubular degeneration. Quantification of tubular pathology and NGAL immunolabeling (D) ( n = 6 kidneys). (E) Relative metabolite levels in kidneys of indicated mice harvested 21 days post tamoxifen administration ( n = 9 Aco2 +/+ and n = 8 Aco2 fl/fl ). (F) Quantification of p-eIF2α relative to total eIF2α from immunoblot of kidneys harvested 21 days post tamoxifen administration, shown in ( n = 6 Aco2 +/+ and n = 7 Aco2 fl/fl ). (G) Gene expression of selected ATF4 target and proximal tubule cell marker genes in kidneys harvested 21 days post tamoxifen administration ( n = 9 Aco2 +/+ and n = 8 Aco2 fl/fl ). Data are mean ± SD with independent replicates as indicated for each graph. Significance was assessed using an unpaired two-tailed t test (A and F), a two-way ANOVA with Sidak’s multiple-comparisons post-test relative to Aco2 +/+ control (B, E, and G), or Fisher’s exact test (D). See also .

Article Snippet: Phospho-eIF2 alpha (Ser51) (119A11) Rabbit Monoclonal Antibody , Cell Signaling Technology , Cat#3597; RRID: AB_390740.

Techniques: Concentration Assay, Immunohistochemistry, Immunolabeling, Western Blot, Gene Expression, Marker, Two Tailed Test, Control

Journal: Veterinary Research

Article Title: The CP312R protein of African swine fever virus inhibits host protein translation via the BiP/PERK/eIF2 α pathway

doi: 10.1186/s13567-025-01688-5

Figure Lengend Snippet: pCP312R promotes the phosphorylation of PERK and eIF2 α . A pCP312R promotes the phosphorylation of PERK in a dose-dependent manner. HEK293T cells were transfected with an increasing amount of pCA-CP312R-Flag (0, 0.5, 1, 1.5, or 2 μg). At 24 h post-transfection (hpt), the cells were collected for western blotting analysis using anti-Flag, anti-p-PERK (Thr982), anti-PERK, or anti- β -actin antibodies. B pCP312R promotes eIF2 α phosphorylation in a dose-dependent manner. HEK293T cells were transfected with an increasing amount of pCA-CP312R-Flag (0, 0.5, 1, 1.5, or 2 μg). At 12 hpt, the cells were incubated with 300 nM thapsigargin (Tg) for 8 h, and then the cells were collected for western blotting analysis using anti-Flag, anti-p-eIF2 α , anti-eIF2 α , or anti- β -actin antibodies. C Inhibition of the cellular protein synthesis by pCP312R is attenuated by ISRIB. HEK293T cells were transfected with an increasing amount of pCA-CP312R-Flag (0, 1, 1.5, 2, or 2.5 μg). At 12 hpt, the cells were treated with ISRIB (0.5 μM) for another 12 h. The cells were pulsed with 3 μM puromycin for 30 min, followed by western blotting analysis using anti-Flag, anti-puromycin, or anti- β -actin antibodies.

Article Snippet: Mouse anti-p72 monoclonal antibody (MAb) (catalog no. Anti PPA 1BC11, INGENASA), mouse anti-puromycin MAb (catalog no. MABE343, Sigma-Aldrich), rabbit anti- β -actin PAbs (catalog no. AC026, ABclonal), mouse anti-Flag MAb (catalog no. M8823, Sigma-Aldrich), mouse anti-GST MAb (catalog no. K200006M, Solarbio), rabbit anti-Myc PAbs (catalog no. YN5506, ImmunoWay), rabbit anti-KDEL PAbs (catalog no. ab214714, Abcam), rabbit anti-BiP PAbs (catalog no. A0241, ABclonal), mouse anti-PERK MAb (catalog no. 3192S, CST), rabbit anti-p-PERK PAbs (catalog no. BS66100, Bioworld), rabbit anti-p-eIF2 α PAbs (catalog no. 3398S, CST), and rabbit anti-eIF2 α PAbs (catalog no. 9722S, CST) are commercially available.

Techniques: Phospho-proteomics, Transfection, Western Blot, Incubation, Inhibition